HL-60 cell line is a human myeloid leukemia cell line, which was originally derived from the peripheral blood of a patient with acute promyelocytic leukemia. HL-60 cells are widely used in research to study the mechanisms of leukemogenesis, cell differentiation, and drug discovery for various hematological malignancies.

HL-60 cells are characterized by their ability to differentiate into various myeloid lineages, including granulocytes, monocytes, and macrophages, in response to various differentiation agents. They are widely used in the study of myeloid cell biology, signaling pathways, and cellular responses to various stimuli, including cytokines and chemotherapeutic agents.

HL-60 cells are also used as a model system to screen potential therapeutic agents for various hematological malignancies, including leukemia, lymphoma, and myeloma. Researchers use HL-60 cells to investigate the molecular mechanisms underlying drug resistance and to identify novel targets for therapeutic intervention.

It is important to handle and culture HL-60 cells using appropriate techniques and conditions to ensure their optimal growth and viability. These include maintaining the cells in suitable culture medium, providing the appropriate temperature and humidity, and regularly monitoring cell confluence and viability.