The EL4 cell line is a murine (mouse-derived) T lymphocyte cell line, which is frequently used in immunological research. It was originally derived from a spontaneous lymphoma that arose in a C57BL/6 mouse. The EL4 cells exhibit characteristics of mature T cells and are known for their ability to produce cytokines such as interleukin-2 (IL-2) and interferon-gamma (IFN-γ).

EL4 cells are widely used to study T cell biology, immune responses, and signaling pathways. Researchers also use EL4 cells to investigate molecular mechanisms involved in cancer, inflammation, and autoimmune diseases, as well as to screen potential therapeutic agents for these conditions.

Since the EL4 cell line is of mouse origin, it serves as an excellent in vitro model system for studying murine T cell biology and can be used in conjunction with in vivo mouse models to generate comprehensive data on the immune system and T cell-related pathologies.

When culturing EL4 cells, it is essential to maintain appropriate culture conditions, such as using suitable culture medium, maintaining the correct temperature and humidity, and regularly monitoring cell confluence and viability.